Nikon Spinning Disk Confocal Microscope

Haworth Hall 1043 bay 2

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Eclipse Ti2-E Inverted Microscope
- Ultra-wide 25mm field of view, fully motorized and intelligent
- Perfect Focus System for real-time drift correction
Crest Optics X-Light V3 spinning disk confocal (Disc pattern: 50um pinholes/250um spacing)
Lumencor Celesta Light Engine
- Excitation (Ex)/ Emission (Em) filters:
  - 365/438 (DAPI; Hoechst)
  - 488/511 nm (FITC, GFP, Alexa Fluor 488)
  - 561/595 nm (Cy3, Alexa Flour 555, 568, Texas Red)
  - 640/684 nm (Cy5, Alexa Fluor 647; 660)
  - 730/797 nm (Cy7, NIR, Alexa Fluor 750)
- Other Ex/Em filters combined
  - 440/485 nm (CFP)
  - 514/560 nm (YFP)
Photometrics KINETIX 10MP CMOS camera (3200 x 3200; 6.5 um pixel size)
- 8-bit speed, 12-bit sensitivity, 16-bit dynamic range,16-bit sub electron
- 25mm (2720 x 2720), 22mm (2394 x 2394), 2048 x 2048, 1024 x1024, and 512 x 512 pixels resolution
Fluorescent D-LEDI light with quad-pass filter set for DAPI, FITC, TRIC and Cy5
Additional modalities:
- Brightfield/ DIC
- iLAS2 TIRF/ Photostimulation illuminator
  - Multiple TIRF options (circle - single/multi angle, arc, or single point TIRF)
  - 405 nm STIM, 488 nm, 561 nm, 640 nm
Tokai Hit Incubation System with Enclosure and Stage-top Unit
- Dark type warming box incubator for CO2 gas, temperature control and stage adapter

Objective Lenses Specifications: Magnification, Numerical Aperture, and Optical Properties
Magnification	Numerical Aperture	Corrections/Properties/Immersion Medium
10X	0.45	NIKON PLAN APO λD OFN25/ DIC
20X	0.80	NIKON PLAN APO λD 20X OFN25/ DIC
40X	0.95	NIKON PLAN APO λD 40X OFN25/ DIC
60X	1.42	NIKON PLAN APO λD OFN25/ OIL
100X	1.45	NIKON PLAN APO λD OFN25/ OIL
60X TIRF	1.49	NIKON APO TIRF/ DIC N2/ OIL