Shimadzu High Performance Liquid Chromatography (HPLC) System

Gray-Little Hall, Room B105

Questions?

Please send a message to mai-mctlab@ku.edu

System controller: SCL-40
Degassing unit: DGU-405
Solvent delivery module: LC-40D
Autosampler: SIL-40C
Column oven: CTO-40C
Detectors:
- Photodiode Array Detector (SPD-M40)
- Refractive Index Detector (RID-20A)

Features a needle in the flow path design to minimize carryover.
Standard sample loop (100 µL)
Total injection method: 0.1 to 100 µL in increments of 0.1 µL from 0.1 to 9.9 µL, and 1 µL from 10-50 µL.
Performs sample pretreatment including dilution, reagent addition, co-injection of samples, stacked injection, and overlapped injection.
Temperature control from 4 to 45 °C (± 2 °C accuracy,) with a forced air cooling mechanism to reduce condensation.
Industry-leading cycle time of ≤ 7 seconds.
Injection volume repeatability: RSD ≤1.0 % for 0.5-0.9 µL, RSD ≤0.5 % for 1.0-1.9 µL, RSD ≤0.25 % for 2.0-4.9 µL, RSD ≤0.15 % for injections ≥5.0 µL.
Injection volume accuracy of ±1% for 5 μL injections (n = 20).
Carryover performance: 0.0025 % (no needle rinsing) and 0.0005 % (with rinse).
Equipped with a static rinse port to rinse the outside of the needle before and/or after aspirating sample.
Maximum pressure rating of 44 MPa (440 bar).

Number of channels: 5
Degassing performance: When a UV absorption detector with wavelength of 210 nm and sensitivity of 0.5 AU is used and methanol saturated with dissolved oxygen (HPLC grade at 25 °C) is flowed at a rate of 1ml/min, UV absorption difference with or without the degassing unit is 300 mAU or more.
Maximum operating flow rate: 10 ml/min per channel
Internal capacity: 400 µl/1channel
Pressure resistance: ±0.1 MPa (only for suction with the solvent delivery module)

Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline.
Flow rate range from 0.1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision ≤0.06 % RSD or 0.02 min SD of the set flow rate, (whichever is larger).
Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
Maximum pressure of 44 MPa/440 bar (up to 5 mL/min) with pH range of 1-14.
Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation unit (LPGE) can be added as options.

Temperature control range is (ambient - 10) °C to 100 °C and the temperature setting range is 4 °C to 100 °C in 1 °C steps.
Temperature accuracy is ±0.5 °C.
Temperature control precision is ± 0.05 °C.
The oven can store at least 6 columns of 150 mm length or 3 columns of 300 mm length.
Unit dimensions is 260 mm x 415 mm x 500 mm (WxHxD).