3i/ Zeiss Two (2)- Photon Upright Microscope (Under Development)

3i/ Zeiss Two (2)- Photon Upright Microscope
Haworth Hall Room 1043 Bay 2


Eduardo Rosa-Molinar, Ph.D

Covid Update

We kindly ask all MAI staff and visitors to adhere to the latest COVID-19 health and safety guidelines on campus. Thank you for prioritizing everyone's well-being.


  • Zeiss Axio Examiner.Z1 fully motorized fixed stage upright two-photon excitation microscope workstation with a Vector™ scan head with dual galvo scanning
  • mBeam™ remote beam expansion
  • Pockels cell modulator
  • Beam delivery optics and shielding
  • Four GaAsP PMTs with automatic overload and computer-controlled reset
  • Complete filter set for multiphoton including dichroics and emission filters for simultaneous multicolor imaging
    • Examiner slider: 750 edge
    • Nosepiece input: 680 SP
    • Nosepiece cube: 445/45,495 edge,525/40
    • TFP/YFP: 520 LP edge, 494/34, 542/27
    • TFP/YFP Wide: 520 LP edge, 488/50, 550/49
    • Cactus input: 720 SP: Cactus cube, red/far red 617/73, 660 edge, 692/40
  • Additional filter cube for polarized light imaging
    • Cactus cube, polarized light: 525/40, s polarizer, 529/23 polarizing bandpass, p polarizer
  • Rapid 90° switching of the polarization of the excitation beam
  • Piezo objective focusing collar with a 400μm range for high-speed volumetric imaging
  • Microscope filter cube for GFP for viewing epifluorescence by eye
  • Motorized ZDeck system with ProScan XY stage
  • ProScan III controller
  • Joystick
  • Universal Slide holder
  • Solid aluminum plate and pillar spacers for Zeiss Axio Examiner.Z1
  • X-Cite 120LED mini white light LED system with direct coupler for Zeiss Axio Examiner.Z1
  • W Plan-Apochromat 20x/1.0NA water dipping objective for Zeiss Axio Examiner.Z1; working distance 2.4mm, extended IR transmission at least 60% at 1300 nm
  • 48" x 60" x 4.5" aluminum optical (breadboard top) vibration-isolation table
  • Fully interlocked laser safety enclosure
  • The two-photon excitation microscope workstation is equipped with the Chameleon Discovery, a versatile, hands-free, and fully integrated ultra-fast femtosecond laser system with a complete tuning range of 680-1300 nm. The Chameleon Discovery laser has a second output that is a fixed 1040 line, which has been combined with the tunable line to accommodate dual 2P excitation. 


Objective Lenses Specifications: Magnification, Numerical Aperture, and Optical Properties
MagnificationNumerical ApertureCorrections/Properties/Immersion Medium
20X1.0W Plan-Apochromat / Zeiss Axio Examiner.Z1 / Water Dipping / IR 60% at 1300 nm / 2.4mm WD*

*Specialized objectives available upon request.

In Vivo Animal Imaging

  • V-1 tabletop from VetEquip Inc.
  • Isoflurane vaporizer with automatic flowrate and temperature compensation capability in continuous flow of inhalation anesthesia.
  • Pure oxygen regulated by a flowmeter (0-4 LPM).
  • V-1 system and flowmeter connect to a 2-liter vented induction chamber where the specimen is exposed to anesthetic vapor and oxygen until unconsciousness.
    • Dimensions and specifications: 13.0" x 5.35" x 6.0" overall size; Induction Chamber size: 10.0" x 5.5" with a ceiling height that slopes from 4.2" to 3.2"; sliding lid; 15mm ID x 22mm OD gas inlet; 30mm OD exhaust port.
  • Vapor Guard TM activated charcoal adsorption filter for waste gas control.
  • Nikon SMZ 445 Stereo Zoom Microscope 0.8x - 3.5x

WorkStation and Support Station Specifications


  • Fully integrated dual six-core computer workstation
  • 32GB RAM
  • SlideBookTM software


  • Intel Zeon E5-2620 processor
  • 64GB RAM
  • NVIDIA Quadro M2000 graphics card
  • one-512 SATA HDD
  • Windows 10 64 bit