3i/Olympus Epifluorescence Inverted Microscope

3i/Olympus Epifluorescence Inverted Microscope
Haworth Hall Room 1043


Eduardo Rosa-Molinar, Ph.D

Covid Update

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  • Olympus IX-81 Inverted Microscope
  • ZDC Prior H117 with Proscan III stage
  • Andor Zyla 4.2 CMOS camera
  • Equipped with dual excitation sources, a Lambda LS Xenon 300W and a white LED coupled to 3 Automated Lambda 10-3 filter wheel (1 excitation, 2 emission)
  • Excitation (Ex)/ Emission (Em) filters:
    • Ex 384/ Em 440/40 (DAPI, Hoechst)
    • Ex 485/ Em 525/30 (FITC, GFP, Alexa Fluor 488)
    • Ex 561/ Em 607/36 (Cy3, Alexa Flour55, 568 or 594, Texas Red)
    • Ex 650/ Em 684/24 (Cy5, APC, Alexa Fluor 647)
    • Brightfield
    • *Other filters may be available upon request.
  • Capture types:
    • 2D and 3D a 2048 x 2048 pixels resolution
    • Timelapse
    • Single and Multiple XY location with autofocus adjustment mode
  • Microscope enclosure equipped with Temperature, Humidity, and CO2 control.
  • Available Olympus Infinity Corrected Objectives

Workstation Specifications

  • SlideBook 6.0 Imaging Software
  • Intel i7-6800k processor
  • 32GB RAM
  • NVIDIA Quadro K620 graphics card
  • two-256GB SSD RAID
  • one-2TB SATA HDD
  • Windows 10/ 64 bit


Objective Lenses Specifications: Magnification, Numerical Aperture, and Optical Properties
MagnificationNumerical ApertureCorrections/Properties/Immersion Medium
4X0.13Olympus UPlan FL N
10X0.30Olympus UPlan FL N/ Phase1*
10X0.30Olympus UPlan FL N
20X0.45Olympus LUCPlanFL N*
20X0.50Olympus UPlan FL N
20X0.75Olympus UPlanSApo*
40X0.60Olympus LUCPlanFL N/ Phase2
40X0.95Olympus UPlanSApo*
40X0.95Olympus UPlanSApo/ DIC*
40X1.30Olympus LUCPlanFLN/ Oil*
60X0.70Olympus LUCPlanFLN
60X0.90Olympus UPlanApo*
60X0.90Olympus UPlanApo/ DIC*
60X1.42Olympus PlanApo N/ Oil*
100X1.40Olympus UPlanSApo/ Oil*
150X1.45Olympus UApoN/ Oil*

*Specialized objectives available upon request.