3i/Olympus Epifluorescence Inverted Microscope

Haworth Hall Room 1043

Questions?

Eduardo Rosa-Molinar, Ph.D
Director/Professor
mailab@ku.edu

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Olympus IX-81 Inverted Microscope
ZDC Prior H117 with Proscan III stage
Andor Zyla 4.2 CMOS camera
Equipped with dual excitation sources, a Lambda LS Xenon 300W and a white LED coupled to 3 Automated Lambda 10-3 filter wheel (1 excitation, 2 emission)
Excitation (Ex)/ Emission (Em) filters:
- Ex 384/ Em 440/40 (DAPI, Hoechst)
- Ex 485/ Em 525/30 (FITC, GFP, Alexa Fluor 488)
- Ex 561/ Em 607/36 (Cy3, Alexa Flour55, 568 or 594, Texas Red)
- Ex 650/ Em 684/24 (Cy5, APC, Alexa Fluor 647)
- Brightfield
- *Other filters may be available upon request.
Capture types:
- 2D and 3D a 2048 x 2048 pixels resolution
- Timelapse
- Single and Multiple XY location with autofocus adjustment mode
Microscope enclosure equipped with Temperature, Humidity, and CO2 control.
Available Olympus Infinity Corrected Objectives

Objective Lenses Specifications: Magnification, Numerical Aperture, and Optical Properties
Magnification	Numerical Aperture	Corrections/Properties/Immersion Medium
4X	0.13	Olympus UPlan FL N
10X	0.30	Olympus UPlan FL N/ Phase1*
10X	0.30	Olympus UPlan FL N
20X	0.45	Olympus LUCPlanFL N*
20X	0.50	Olympus UPlan FL N
20X	0.75	Olympus UPlanSApo*
40X	0.60	Olympus LUCPlanFL N/ Phase2
40X	0.95	Olympus UPlanSApo*
40X	0.95	Olympus UPlanSApo/ DIC*
40X	1.30	Olympus LUCPlanFLN/ Oil*
60X	0.70	Olympus LUCPlanFLN
60X	0.90	Olympus UPlanApo*
60X	0.90	Olympus UPlanApo/ DIC*
60X	1.42	Olympus PlanApo N/ Oil*
100X	1.40	Olympus UPlanSApo/ Oil*
150X	1.45	Olympus UApoN/ Oil*

*Specialized objectives available upon request.